نوع مقاله : مقاله کامل علمی پژوهشی
نویسندگان
1 نظارت بر بهداشت مواد غذایی ، دانشکده دامپزشکی، شهرکرد،ایران
2 گروه بهداشت مواد غذایی، دانشکده دامپزشکی، دانشگاه آزاد اسلامی واحد شهرکرد، شهرکرد، ایران
چکیده
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Background and purpose: Mycotoxins are secondary metabolites that are naturally produced by molds and cause contamination of human and livestock food. Aflatoxins are toxic substances that can cause acute and chronic human poisoning with teratogenic effects. They are toxic substances produced by the secondary metabolism of several fungal species such as Aspergillus flavus, Aspergillus parasiticus and Aspergillus nomius, which grow in major food crops under a wide range of climatic conditions. Aflatoxin M1 contamination in milk is a growing concern. Milk is a very nutritious food that has protein, fat, micronutrients and water in its composition, and its contamination with aflatoxin is not far from expected. Considering the high toxicity of aflatoxin M1, and the harmful effects on human health, its measurement in milk is of great importance. The aim of this study was to investigate the occurrence of aflatoxin M1 in ruminant milk supplied in Karaj Counti.
Materials and methods: 60 raw milk samples in summer and autumn seasons (including 20 cow milk samples, 10 buffalo milk samples, 10 camel milk samples, 10 sheep milk samples, 10 goat milk samples) from the supply centers of these products in Karaj Counti was randomly sampled and to evaluate the amount of aflatoxin M1 by ELISA according to the instructions of the AFM1 ELISA kit (Biopharm-R, Germany) was used to measure aflatoxin M1 in milk. They were transferred to the food hygiene and quality control laboratory of the Faculty of Veterinary Medicine of Shahrekord Islamic Azad University. Statistical analysis was performed to determine the differences in the amount of AFM between milk samples in different ruminants using one-way ANOVA and Duncan method. The mean difference was measured at the significance level of p<05.00. Excel software was used to draw graphs.
Findings: The results showed that aflatoxin M1 was detected in all raw milk samples, including cow, sheep, goat, buffalo and camel milk. 29 samples (48.3 %) have contamination less than 5 ng/kg, 27 samples (45 %) have contamination in the range of 20-5 ng/liter, and 4 samples (6.70 %) in the range of 20-50 ng/liter were contaminated with aflatoxin. Also, in this study, none of the raw milk samples of ruminants had contamination beyond 50 ng/liter.
Conclusion: The results showed that none of the aflatoxins in the present study exceeded the standard. due to lower pollution than Iran's standard; Europe and America In the present study, the use of ruminant milk sold in Karaj Counti cannot pose any risk to the health of consumers.
کلیدواژه‌ها [English]
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