بهینه سازی فرایند الکتروریسی نانوحامل پلی (وینیل الکل) حاوی عصاره‌ ترش‌واش (Oxalis corniculata Linn)

نوع مقاله : مقاله کامل علمی پژوهشی

نویسندگان

1 دانشجو دکتری، گروه علوم و صنایع غذایی، پردیس بین المللی ارس، دانشگاه تهران، تهران، ایران

2 استاد، گروه علوم و مهندسی صنایع غذایی، دانشکده مهندسی و فناوری کشاورزی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران

3 دانش آموخته دکتری، گروه علوم و مهندسی صنایع غذایی، دانشکده مهندسی و فناوری کشاورزی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران

چکیده

سابقه و هدف: در حال حاضر روش الکتروریسی تنها روش کارآمد تولید الیاف پیوسته با قطرهای کمتر از چند نانومتر است. این روش در محدوده وسیعی از پلیمرهای مصنوعی و طبیعی استفاده می‌شود. امکان تولید الیافی حاوی ساختار پیچیده مانند هسته-پوسته یا الیاف توخالی نیز با روش‌های ویژه‌ی الکتروریسی وجود دارد. همچنین این روش امکان تولید الیاف تصادفی و موازی را دارا است. تولید نانوحامل‌های یکنواخت با مورفولوژی و قطر خاص تحت تأثیر شرایط الکتروریسی است. در همین حال، کنترل متغیرهای مختلف درگیر با فرآیند الکتروریسی کار آسانی نیست، از این رو در تحقیق حاضر روش‌شناسی سطح پاسخ با بهینه‌سازی اثرات متغیرهای مستقل روش الکتروریسی و تولید نانوحامل‌های پلی (وینیل الکل) حاوی عصاره‌ ترش‌واش برای بهبود پایداری، حلالیت و افزایش شانس تحویل عصاره مورد مطالعه قرار گرفته است.
مواد و روش ها: در این تحقیق از دستگاه الکتروریسی برای انکپسولاسیون عصاره Oxalis corniculata Linn (OCEX) با استفاده از پلی وینیل الکل (PVA) استفاده شد. برای مدل‌سازی و بهینه‌سازی از روش آماری سطح پاسخ (RSM) با متغیرهای ورودی شامل غلظت OCEX (5/4 – 5/1 درصد حجمی)، ولتاژ اعمال شده (25 – 15 کیلوولت)، دبی پمپ (1 – 5/0 میلی‌لیتر بر ساعت) و فاصله بین نوک سوزن تا جمع کننده (10 تا 20 سانتی‌متر) استفاده گردید.
یافته ها: طرح مرکب مرکزی (CCD) جهت اجرا و تجزیه و تحلیل مدل استفاده شد آنالیز ریخت‌شناسی توسط میکروسکوپ الکترونی روبشی (SEM) نتایج بهینه‌سازی الکتروریسی را تأیید نمود. بر این اساس شرایط بهینه برای ساخت نانوالیاف عملکردی PVA بارگذاری شده با OCEX (PVANF-OCEX) با قطر پیش‌بینی شده - صحت سنجی شده 311 - 2/320 نانومتر شامل غلظت 5/2 درصد حجمی OCEx، فاصله 5/16 سانتی‌متری بین نوک سوزن تا جمع کننده، ولتاژ اعمالی 5/21 کیلوولتی و نرخ پمپ 7/0 میلی‌لیتر بر ساعت ارزیابی شد.
یافته ها: طرح آماری مرکب مرکزی (CCD) جهت اجرا و تجزیه و تحلیل مدل استفاده شد آنالیز ریخت‌شناسی توسط میکروسکوپ الکترونی روبشی (SEM) نتایج بهینه‌سازی الکتروریسی را تأیید نمود. بر این اساس شرایط بهینه برای ساخت نانوالیاف عملکردی PVA بارگذاری شده با OCEX (PVANF-OCEX) با قطر پیش‌بینی شده - صحت سنجی شده 311 - 2/320 نانومتر شامل غلظت 5/2 درصد حجمی OCEx، فاصله 5/16 سانتی‌متری بین نوک سوزن تا جمع کننده، ولتاژ اعمالی 5/21 کیلوولتی و نرخ پمپ 7/0 میلی‌لیتر بر ساعت ارزیابی شد.
نتیجه گیری: تجزیه و تحلیل SEM تأیید کرد که نانوحامل‌های PVANF-OCEX در نقطه بهینه بدون مهره، سطوح همگن، صاف، توزیع نرمال و OCEX با موفقیت با PVA پوشانده شده‌اند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Optimizing the electrospinning process of poly(vinyl alcohol) nanocarrier containing Oxalis corniculata Linn

نویسندگان [English]

  • Esmaeil Ali Mohammadi Fard 1
  • Zahra Emam-Djomeh 2
  • Mohammad Ekrami 3
1 Ph.D. Student, Department of Food Science and Technology, Aras International Campus, University of Tehran, Tehran, Iran
2 Department of Food Science and Engineering, Faculty of Agricultural Engineering and Technology, Campus of Agriculture and Natural Resources, University of Tehran, Karaj, Iran. *Corresponding Author
3 Ph.D. Graduate, Department of Food Science and Engineering, Faculty of Agricultural Engineering and Technology, Campus of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
چکیده [English]

Abstract
Background and purpose: Currently, the electrospinning method is the only efficient method of producing continuous fibers with diameters less than a few nanometers. This method is used in a wide range of synthetic and natural polymers. It is also possible to produce fibers containing a complex structure such as core-shell or hollow fibers with special electrospinning methods. Also, this method has the possibility of producing random and parallel fibers. The production of uniform nanocarriers with specific morphology and diameter is influenced by electrospinning conditions. Meanwhile, controlling the various variables involved in the electrospinning process is not an easy task, therefore, in this research, the response surface methodology was studied by optimizing the effects of the independent variables of the electrospinning method and the production of PVANF-OCEX nanocarriers to improve stability, solubility and increase the chance of extract delivery.
Materials and methods: In this research, an electrospinning machine was used for the encapsulation of Oxalis corniculata Linn (OCEX) extract using polyvinyl alcohol (PVA). For modeling and optimization using the response surface method (RSM) with input variables including OCEX concentration (1.5 - 4.5% v/v), applied voltage (15 - 25 kV), pump flow rate (0.5 - 1 mL/h) and the distance between the tip of the needle and the collector (10 - 20 cm) was used.
Results: Central composite design (CCD) was used to implement and analyze the model. Morphological analysis by scanning electron microscope (SEM) confirmed the results of electrospinning optimization. Based on this, the optimal conditions for the fabrication of functional PVA nanofibers loaded with OCEX (PVANF-OCEX) with a predicted-validated diameter of 320.2-311 nm include a concentration of 2.5% by volume of OCEx, a distance of 16.5 cm between the needle tips. Up to the collector, the applied voltage was 21.5 kV and the pump rate was evaluated as 0.7 ml/hour.
Materials and methods: In this research, an electrospinning machine was used for the encapsulation of Oxalis corniculata Linn (OCEX) extract using polyvinyl alcohol (PVA). For modeling and optimization using the response surface method (RSM) with input variables including OCEX concentration (1.5 - 4.5% v/v), applied voltage (15 - 25 kV), pump flow rate (0.5 - 1 mL/h) and the distance between the tip of the needle and the collector (10 - 20 cm) was used.
Results: Central composite design (CCD) was used to implement and analyze the model. Morphological analysis by scanning electron microscope (SEM) confirmed the results of electrospinning optimization. Based on this, the optimal conditions for the fabrication of functional PVA nanofibers loaded with OCEX (PVANF-OCEX) with a predicted-validated diameter of 320.2-311 nm include a concentration of 2.5% by volume of OCEx, a distance of 16.5 cm between the needle tips. Up to the collector, the applied voltage was 21.5 kV and the pump rate was evaluated as 0.7 ml/hour.
Conclusion: SEM analysis confirmed that the PVANF-OCEX nanocarriers were successfully coated with PVA at the optimum point without beads, homogeneous, smooth, normal distribution and OCEX surfaces.

کلیدواژه‌ها [English]

  • Electrospinning
  • Oxalis corniculata Linn
  • Encapsulation
  • Response Surface Method
  • Optimization

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نشریه فرآوری و نگهداری مواد غذایی
دوره 17، شماره 2
خرداد 1404
صفحه 1-22

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