ارزیابی ویژگی های امولسیون های دوگانه (W/O/W) تثبیت شده با کونژوگه ایزوله پروتئین آب پنیر – اینولین

نوع مقاله : مقاله کامل علمی پژوهشی

نویسندگان

1 دانش آموخته دکتری، گروه علوم و صنایع غذایی، دانشکده صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

2 استاد، گروه علوم و صنایع غذایی، دانشکده صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران (* رایانامه نویسنده مسئول: moghorbani@yahoo.com)

3 استاد، گروه علوم و صنایع غذایی، دانشکده صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

چکیده

چکیده:
سابقه و هدف: گلیکاسیون پروتئین معمولا به‌عنوان مرحله اول واکنش میلارد در نظر گرفته می‌شود، مطالعات انجام گرفته در سال-های اخیر مشخص ساختند که خصوصیات کاربردی ایزوله پروتئین آب پنیر توسط کونژوگه شدن با کربوهیدرات‌ها از طریق فرایند میلارد بهبود چشم‌گیری یافته است. لذا هدف از این پژوهش استفاده از کونژوگه‌های ایزوله پروتئین آب پنیر- اینولین جهت تثبیت امولسیون‌های دوگانه (W/O/W) می‌باشد.
مواد و روش‌ها: در این پژوهش از کونژوگه‌های ایزوله پروتئین آب پنیر- اینولین (نسبت وزنی 1:1، 1:2 و2:1) تولیدی در دمای 60 و 80 درجه سانتی‌گراد جهت تثبیت امولسیون‌های دوگانه (W/O/W) گردید. پروفایل الکتروفورز ژل پلی آکریل آمید (SDS-PAGE) کونژوگه‌های ایزوله پروتئین آب پنیر- اینولین، طیف‌سنجی تبدیل فوریه مادون قرمز (FT-IR) و بررسی رنگ کونژوگه-ها انجام شد و همچنین تعیین اندازه قطرات امولسیون‌ها، پتانسیل زتا، ویسکوزیته و خامه ای شدن امولسیون‌های دوگانه (W/O/W) بررسی گردید.
یافته‌ها: بررسی اندازه قطرات امولسیون‌های دوگانه (W/O/W) نشان داد که متوسط اندازه قطرات امولسیون‌های تثبیت شده با کونژوگه‌های تولیدشده در دمای 80 درجه‌ی سانتی‌گراد کمتر از اندازه قطرات امولسیون‌های پایدارشده با کونژوگه‌های تولیدی در دمای 60 درجه سانتی‌گراد بود. نسبت 2:1 و 1:2 کونژوگه ایزوله پروتئین آب پنیر – اینولین به ترتیب کمترین و بیشترین اندازه قطرات را در روز نخست و بعد از 21 روز نگهداری نشان دادند. نتایج بررسی ویسکوزیته امولسیون‌ها نیز مشخص ساخت که امولسیون‌های دوگانه تثبیت شده با نسبت 2:1 کونژوگه ایزوله پروتئین آب پنیر – اینولین دارای ویسکوزیته کمتری نسبت به سایر امولسیون‌ها بودند. امولسیون‌های تثبیت شده با کونژوگه‌های انکوبه شده در دمای 80 درجه سانتی‌گراد پتانسیل زتا منفی بیشتری نسبت به امولسیون‌های تثبیت شده با کونژوگه‌های انکوبه شده در دمای 60 درجه سانتی‌‌گراد در نسبت‌های یکسان وزنی پروتئین – پلی ساکارید در روز نخست تولید نشان دادند. همچنین امولسیون‌های تثبیت شده با نسبت 1:2 کونژوگه ایزوله پروتئین آب پنیر – اینولین که در دمای 60 و 80 درجه سانتی‌گراد تولید شده بودند، مقدار پتانسیل زتا بیشتری را در روز نخست و بعد از 21 روز نگهداری نشان دادند.
نتیجه‌گیری: به‌طورکلی مشخص شد که کونژوگه های ایزوله پروتئین آب پنیر – اینولین توانایی مطلوبی جهت تثبیت امولسیون‌های دوگانه به خصوص در نسبت وزنی 1:2 دارند، از این رو می‌توان از این امولسیون‌ها جهت ریزپوشانی ترکیبات زیست فعال مختلف استفاده نمود.
چکیده:
سابقه و هدف: گلیکاسیون پروتئین معمولا به‌عنوان مرحله اول واکنش میلارد در نظر گرفته می‌شود، مطالعات انجام گرفته در سال-های اخیر مشخص ساختند که خصوصیات کاربردی ایزوله پروتئین آب پنیر توسط کونژوگه شدن با کربوهیدرات‌ها از طریق فرایند میلارد بهبود چشم‌گیری یافته است. لذا هدف از این پژوهش استفاده از کونژوگه‌های ایزوله پروتئین آب پنیر- اینولین جهت تثبیت امولسیون‌های دوگانه (W/O/W) می‌باشد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Evaluation of the properties of double emulsions (W/O/W) stabilized with whey protein isolate-inulin conjugate

نویسندگان [English]

  • ADELEH MOHAMMADI 1
  • Mohammad Ghorbani 2
  • علیرضا Sadeghi Mahoonak 3
  • seid mahdi Jafari 3
1 PhD student, Department of Food Science and Technology, Faculty of Food Science and Technology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
2 Professor, Department of Food Science and Technology, Faculty of Food Science and Technology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran, )*Corresponding author; Email address: moghorbani@yahoo.com(
3 Professor, Department of Food Science and Technology, Faculty of Food Science and Technology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
چکیده [English]

Abstract:
Background and objectives: The glycation of Proteins usually considered as the first step of the Maillard reaction. Studies have shown that the functional properties of whey protein isolate have been significantly improved by conjugation by carbohydrates through the Millard process. Therefore,the aim of this research is to stibilize double emulsions (W/O/W) with whey protein isolate -inulin conjugates.
Materials and methods: The conjugation variables were WPI to inulin ratios (1:1, 1:2, and 2:1w/w) and incubation temperatures (60 and 80°C) at 79% relative humidity for 24 h. In this study, we investigated the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS- PAGE) , Fourier transform infrared spectroscopy (FT-IR) and color of conjugates. The apparent viscosity, particle size and distribution, zeta potential and creaming of W/O/W emulsions were measured during storage time. Emulsions stabilized by conjugates produced by heating at 80°C had higher zeta potential than emulsions stabilized with conjugates produced by heating at 60°C on the first day of production. The W1/O/W2 emulsions stabilized by 1:2 ratio of whey protein isolate-inulin conjugate showed a higher zeta potential value on the first day and after 21 days of storage.
Results: The droplet size of W/O/W emulsions stabilized with conjugates produced at 80 ° C was less than the conjugates prepared at 60° C. The 2:1 and 1:2 weight ratios of whey protein isolate-inulin showed the maximum and minimum droplet size of emulsions during storage.
Conclusion: Generally, the results showed that whey protein isolate-inulin conjugates have a favorable ability to stabilize double emulsions, especially a weight ratio of 1:2, hence these emulsions can be used for the encapsulation of various bioactive compounds.
Abstract:
Background and objectives: The glycation of Proteins usually considered as the first step of the Maillard reaction. Studies have shown that the functional properties of whey protein isolate have been significantly improved by conjugation by carbohydrates through the Millard process. Therefore,the aim of this research is to stibilize double emulsions (W/O/W) with whey protein isolate -inulin conjugates.
Materials and methods: The conjugation variables were WPI to inulin ratios (1:1, 1:2, and 2:1w/w) and incubation temperatures (60 and 80°C) at 79% relative humidity for 24 h. In this study, we investigated the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS- PAGE) , Fourier transform infrared spectroscopy (FT-IR) and color of conjugates. The apparent viscosity, particle size and distribution, zeta potential and creaming of W/O/W emulsions were measured during storage time. Emulsions stabilized by conjugates produced by heating at 80°C had higher zeta potential than emulsions stabilized with conjugates produced by heating at 60°C on the first day of production. The W1/O/W2 emulsions stabilized by 1:2 ratio of whey protein isolate-inulin conjugate showed a higher zeta potential value on the first day and after 21 days of storage.
Results: The droplet size of W/O/W emulsions stabilized with conjugates produced at 80 ° C was less than the conjugates prepared at 60° C. The 2:1 and 1:2 weight ratios of whey protein isolate-inulin showed the maximum and minimum droplet size of emulsions during storage.
Conclusion: Generally, the results showed that whey protein isolate-inulin conjugates have a favorable ability to stabilize double emulsions, especially a weight ratio of 1:2, hence these emulsions can be used for the encapsulation of various bioactive compounds.

کلیدواژه‌ها [English]

  • " W/O/W emulsions"
  • "Maillard conjugation"
  • "whey protein isolate"
  • "Inulin"
  1. Tan, C., McClements, D.J. 2021. Application of Advanced Emulsion Technology in the Food Industry: A Review and Critical Evaluation. Foods. 10(4): 812.
  2. Vellido-Perez, J., Ochando-Pulido, J., Brito-de la Fuente, E., Martinez-Ferez, A. 2021a. Novel emulsions–based technological approaches for the protection of omega–3 polyunsaturated fatty acids against oxidation processes–A comprehensive review. Food Structure. 100175.
  3. Qi, P.X., Xiao, Y., Wickham, E.D. 2017. Stabilization of whey protein isolate (WPI) through interactions with sugar beet pectin (SBP) induced by controlled dry-heating. Food Hydrocolloids. 67: 1-13.
  4. Wang, C., Wang, H., Sun, X., Sun, Y., Guo, M. 2019. Heat-Induced Interactions between Whey Protein and Inulin and Changes in Physicochemical and Antioxidative Properties of the Complexes. International Journal of Molecular Sciences. 20(17): 4089.
  5. O'Mahony, J.A., Drapala, K.P., Mulcahy, E.M., Mulvihill, D.M. 2017. Controlled glycation of milk proteins and peptides: Functional properties. International Dairy Journal. 67: 16-34.
  6. de Castro, R.J.S., Domingues, M.A.F., Ohara, A., Okuro, P.K., dos Santos, J.G., Brexó, R.P., Sato, H.H. 2017. Whey protein as a key component in food systems: Physicochemical properties, production technologies and applications. Food Structure. 14: 17-29.
  7. Chen, W., Wang, W., Ma, X., Lv, R., Watharkar, R.B., Ding, T., Ye, X., Liu, D. 2019. Effect of pH-shifting treatment on structural and functional properties of whey protein isolate and its interaction with (−)-epigallocatechin-3-gallate. Food Chemistry. 274: 234-241.
  8. Sutariya, S., Patel, H. 2017. Effect of hydrogen peroxide on improving the heat stability of whey protein isolate solutions. Food Chemistry. 223: 114-120.
  9. de Oliveira, F.C., Coimbra, J.S.d.R., de Oliveira, E.B., Zuñiga, A.D.G., Rojas, E.E.G. 2016. Food protein-polysaccharide conjugates obtained via the Maillard reaction: A review. Critical Reviews in Food Science and Nutrition. 56(7): 1108-1125.
  10. Sun, W.-W., Yu, S.-J., Yang, X.-Q., Wang, J.-M., Zhang, J.-B., Zhang, Y., Zheng, E.-L. 2011. Study on the rheological properties of heat-induced whey protein isolate–dextran conjugate gel. Food Research International. 44(10): 3259-3263.
  11. Mulcahy, E.M., Fargier-Lagrange, M., Mulvihill, D.M., O'Mahony, J.A. 2017. Characterisation of heat-induced protein aggregation in whey protein isolate and the influence of aggregation on the availability of amino groups as measured by the ortho-phthaldialdehyde (OPA) and trinitrobenzenesulfonic acid (TNBS) methods. Food Chemistry. 229: 66-74.
  12. Al-Hakkak, J., Al-Hakkak, F. 2010. Functional egg white–pectin conjugates prepared by controlled Maillard reaction. Journal of Food Engineering. 100(1): 152-159.
  13. Evans, M., Ratcliffe, I., Williams, P.A. 2013. Emulsion stabilisation using polysaccharide–protein complexes. Current Opinion in Colloid & Interface Science. 18(4): 272-282.
  14. Wong, B.T., Day, L., Augustin, M.A. 2011. Deamidated wheat protein–dextran Maillard conjugates: Effect of size and location of polysaccharide conjugated on steric stabilization of emulsions at acidic pH. Food Hydrocolloids. 25(6): 1424-1432.
  15. Zhang, J., Wu, N., Lan, T., Yang, X. 2014. Improvement in emulsifying properties of soy protein isolate by conjugation with maltodextrin using high-temperature, short-time dry-heating Maillard reaction. International Journal of Food Science & Technology. 49(2): 460-467.
  16. Tamnak, S., Mirhosseini, H., Tan, C.P., Ghazali, H.M., Muhammad, K. 2016. Physicochemical properties, rheological behavior and morphology of pectin-pea protein isolate mixtures and conjugates in aqueous system and oil in water emulsion. Food Hydrocolloids. 56: 405-416.
  17. Wang, L.-H., Sun, X., Huang, G.-Q., Xiao, J.-X. 2018. Conjugation of soybean protein isolate with xylose/fructose through wet-heating Maillard reaction. Journal of Food Measurement and Characterization. 12(4): 2718-2724.
  18. Zha, F., Dong, S., Rao, J., Chen, B. 2019. The structural modification of pea protein concentrate with gum Arabic by controlled Maillard reaction enhances its functional properties and flavor attributes. Food Hydrocolloids. 92: 30-40.
  19. Song, Y., Yang, S., Li, J. 2020. Effect of Maillard reaction conditions on the solubility and molecular properties of wheat gluten–maltose conjugates. Food science & nutrition. 8: 5898-5906.
  20. Giarnetti, M., Paradiso, V.M., Caponio, F., Summo, C., Pasqualone, A. 2015. Fat replacement in shortbread cookies using an emulsion filled gel based on inulin and extra virgin olive oil. LWT - Food Science and Technology. 63(1): 339-345.
  21. Leyva-Porras, C., López-Pablos, A., Alvarez-Salas, C., Perez-Urizar, J., Saavedra-Leos, Z. 2015. Physical properties of inulin and technological applications. Polysaccharides; Ramawat, KG, Mérillon, JM, Eds.; Springer: New York, NY, USA. 959-984.
  22. Schmidt, U.S., Pietsch, V.L., Rentschler, C., Kurz, T., Endreß, H.U., Schuchmann, H.P. 2016. Influence of the degree of esterification on the emulsifying performance of conjugates formed between whey protein isolate and citrus pectin. Food Hydrocolloids. 56: 1-8.
  23. Mohammadi, A., Jafari, S.M., Assadpour, E., Faridi Esfanjani, A. 2016. Nano-encapsulation of olive leaf phenolic compounds through WPC–pectin complexes and evaluating their release rate. International Journal of Biological Macromolecules. 82: 816-822.
  24. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227: 680.
  25. Pirestani, S., Nasirpour, A., Keramat, J., Desobry, S., Jasniewski, J. 2018. Structural properties of canola protein isolate-gum Arabic Maillard conjugate in an aqueous model system. Food Hydrocolloids. 79: 228-234.
  26. Martins, J.T., Cerqueira, M.A., Bourbon, A.I., Pinheiro, A.C., Souza, B.W., Vicente, A.A. 2012. Synergistic effects between κ-carrageenan and locust bean gum on physicochemical properties of edible films made thereof. Food Hydrocolloids. 29(2): 280-289.
  27. Yang, Y., Cui, S., Gong, J., Miller, S.S., Wang, Q., Hua, Y. 2015. Stability of citral in oil-in-water emulsions protected by a soy protein–polysaccharide Maillard reaction product. Food Research International. 69: 357-363.
  28. Gómez-Ordóñez, E., Rupérez, P. 2011. FTIR-ATR spectroscopy as a tool for polysaccharide identification in edible brown and red seaweeds. Food Hydrocolloids. 25(6): 1514-1520.
  29. Zhu, X., Jia, C., Meng, X., Xing, M., Yi, Y., Gao, X. 2018. Synthesis, characterization of inulin propionate ester, and evaluation of its in vitro effect on SCFA production. Starch‐Stärke. 70(9-10): 1800037.
  30. Barth, A. 2007. Infrared spectroscopy of proteins. Biochimica et Biophysica Acta (BBA)-Bioenergetics. 1767(9): 1073-1101.
  31. Sadeghi, S., Madadlou, A., Yarmand, M. 2014. Microemulsification–cold gelation of whey proteins for nanoencapsulation of date palm pit extract. Food Hydrocolloids. 35: 590-596.
  32. Ozel, B., Aydin, O., Oztop, M.H. 2020. In vitro digestion of polysaccharide including whey protein isolate hydrogels. Carbohydrate Polymers. 229: 115469.
  33. Lefèvre, T., Subirade, M. 1999. Structural and interaction properties of β-Lactoglobulin as studied by FTIR spectroscopy. International Journal of Food Science & Technology. 34(5-6): 419-428.
  34. Ye, M., Zhou, R., Shi, Y., Chen, H., Du, Y. 2017. Effects of heating on the secondary structure of proteins in milk powders using mid-infrared spectroscopy. Journal of Dairy Science. 100(1): 89-95.
  35. González-Martínez, D., Carrillo-Navas, H., Barrera-Díaz, C., Martínez-Vargas, S., Alvarez-Ramírez, J., Pérez-Alonso, C. 2017. Characterization of a novel complex coacervate based on whey protein isolate-tamarind seed mucilage. Food Hydrocolloids. 72: 115-126.
  36. Shi, Y., Liang, R., Chen, L., Liu, H., Goff, H.D., Ma, J., Zhong, F. 2019. The antioxidant mechanism of Maillard reaction products in oil-in-water emulsion system. Food Hydrocolloids. 87: 582-592.
  37. Nooshkam, M., Madadlou, A. 2016. Microwave-assisted isomerisation of lactose to lactulose and Maillard conjugation of lactulose and lactose with whey proteins and peptides. Food Chemistry. 200: 1-9.
  38. Dong, X., Du, S., Deng, Q., Tang, H., Yang, C., Wei, F., Chen, H., Quek, S.Y., Zhou, A., Liu, L. 2020. Study on the antioxidant activity and emulsifying properties of flaxseed gum-whey protein isolate conjugates prepared by Maillard reaction. International Journal of Biological Macromolecules. 153: 1157-1164.
  39. Apolinário, A.C., de Carvalho, E.M., de Lima Damasceno, B.P.G., da Silva, P.C.D., Converti, A., Pessoa Jr, A., da Silva, J.A. 2017. Extraction, isolation and characterization of inulin from Agave sisalana boles. Industrial Crops and Products. 108: 355-362.
  40. Carpenter, J.F., Crowe, J.H. 1989. An infrared spectroscopic study of the interactions of carbohydrates with dried proteins. Biochemistry. 28(9): 3916-3922.
  41. Tan, S., Zhong, C., Langrish, T. 2020. Pre-gelation assisted spray drying of whey protein isolates (WPI) for microencapsulation and controlled release. LWT. 117: 108625.
  42. Nasrollahzadeh, F., Varidi, M., Koocheki, A., Hadizadeh, F. 2017. Effect of microwave and conventional heating on structural, functional and antioxidant properties of bovine serum albumin-maltodextrin conjugates through Maillard reaction. Food Research International. 100: 289-297.
  43. Barbosa, J.M., Ushikubo, F.Y., de Figueiredo Furtado, G., Cunha, R.L. 2019. Oil in water emulsions stabilized by maillard conjugates of sodium caseinate-locust bean gum. Journal of Dispersion Science and Technology. 40(5): 634-645.
  44. Einhorn-Stoll, U., Kastner, H., Drusch, S. 2014. Thermally induced degradation of citrus pectins during storage–Alterations in molecular structure, colour and thermal analysis. Food Hydrocolloids. 35: 565-575.
  45. Perrechil, F., Santana, R., Lima, D., Polastro, M., Cunha, R. 2014. Emulsifying properties of maillard conjugates produced from sodium caseinate and locust bean gum. Brazilian Journal of Chemical Engineering. 31(2): 429-438.
  46. Liu, G., Zhong, Q. 2015. High temperature-short time glycation to improve heat stability of whey protein and reduce color formation. Food Hydrocolloids. 44: 453-460.
  47. McClements, D.J., (2004). Food emulsions: principles, practices, and techniques. CRC press.
  48. Zhou, Y., Teng, F., Tian, T., Sami, R., Wu, C., Zhu, Y., Zheng, L., Jiang, L., Wang, Z., Li, Y. 2020. The impact of soy protein isolate-dextran conjugation on capsicum oleoresin (Capsicum annuum L.) nanoemulsions. Food Hydrocolloids. 108: 105818.
  49. Weiss, J., Muschiolik, G. 2007. Factors affecting the droplet size of water‐in‐oil emulsions (W/O) and the oil globule size in water‐in‐oil‐in‐water emulsions (W/O/W). Journal of Dispersion Science and Technology. 28(5): 703-716.
  50. Mutaliyeva, B., Grigoriev, D., Madybekova, G., Sharipova, A., Aidarova, S., Saparbekova, A., Miller, R. 2017. Microencapsulation of insulin and its release using w/o/w double emulsion method. Colloids and Surfaces A: Physicochemical and Engineering Aspects. 521: 147-152.
  51. Pagano, A.P.E., Khalid, N., Kobayashi, I., Nakajima, M., Neves, M.A., Bastos, E.L. 2018. Microencapsulation of betanin in monodisperse W/O/W emulsions. Food Research International. 109: 489-496.
  52. Wilde, P.J. 2019. Improving Emulsion Stability Through Selection of Emulsifiers and Stabilizers. Reference Module in Food Science.
  53. Amid, B.T., Mirhosseini, H. 2014. Stabilization of water in oil in water (W/O/W) emulsion using whey protein isolate-conjugated durian seed gum: Enhancement of interfacial activity through conjugation process. Colloids and Surfaces B: Biointerfaces. 113: 107-114.
  54. Delfanian, M., Razavi, S.M.A., Haddad Khodaparast, M.H., Esmaeilzadeh Kenari, R., Golmohammadzadeh, S. 2018. Influence of main emulsion components on the physicochemical and functional properties of W/O/W nano-emulsion: Effect of polyphenols, Hi-Cap, basil seed gum, soy and whey protein isolates. Food Research International. 108: 136-143.
  55. Flores-Andrade, E., Allende-Baltazar, Z., Sandoval-González, P.E., Jiménez-Fernández, M., Beristain, C.I., Pascual-Pineda, L.A. 2021. Carotenoid nanoemulsions stabilized by natural emulsifiers: Whey protein, gum Arabic, and soy lecithin. Journal of Food Engineering. 290: 110208.
  56. Danaei, M., Dehghankhold, M., Ataei, S., Hasanzadeh Davarani, F., Javanmard, R., Dokhani, A., Khorasani, S., Mozafari, M. 2018. Impact of particle size and polydispersity index on the clinical applications of lipidic nanocarrier systems. Pharmaceutics. 10(2): 57.
  57. Bonnet, M., Cansell, M., Berkaoui, A., Ropers, M.-H., Anton, M., Leal-Calderon, F. 2009. Release rate profiles of magnesium from multiple W/O/W emulsions. Food Hydrocolloids. 23(1): 92-101.
  58. Leiva-Vega, J., Villalobos-Carvajal, R., Ferrari, G., Donsì, F., Zúñiga, R.N., Shene, C., Beldarraín-Iznaga, T. 2020. Influence of interfacial structure on physical stability and antioxidant activity of curcumin multilayer emulsions. Food and Bioproducts Processing. 121: 65-75.
  59. O’Regan, J., Mulvihill, D.M. 2010. Sodium caseinate–maltodextrin conjugate stabilized double emulsions: Encapsulation and stability. Food Research International. 43(1): 224-231.
  60. Lutz, R., Aserin, A., Wicker, L., Garti, N. 2009a. Double emulsions stabilized by a charged complex of modified pectin and whey protein isolate. Colloids and Surfaces B: Biointerfaces. 72(1): 121-127.
  61. Sun, C., Gunasekaran, S., Richards, M.P. 2007. Effect of xanthan gum on physicochemical properties of whey protein isolate stabilized oil-in-water emulsions. Food Hydrocolloids. 21(4): 555-564.
  62. Yildirim, M., Sumnu, G., Sahin, S. 2017. The effects of emulsifier type, phase ratio, and homogenization methods on stability of the double emulsion. Journal of Dispersion Science and Technology. 38(6): 807-814.
  63. Herzi, S., Essafi, W., Bellagha, S., Leal-Calderon, F. 2014. Influence of the inner droplet fraction on the release rate profiles from multiple W/O/W emulsions. Colloids and Surfaces A: Physicochemical and Engineering Aspects. 441: 489-495.
  64. Liu, J., Liu, W., Salt, L.J., Ridout, M.J., Ding, Y., Wilde, P.J. 2018b. Fish oil emulsions stabilized with caseinate glycated by dextran: Physicochemical stability and gastrointestinal fate. Journal of Agricultural and Food Chemistry. 67(1): 452-462.
  65. Giroux, H.J., Constantineau, S., Fustier, P., Champagne, C.P., St-Gelais, D., Lacroix, M., Britten, M. 2013. Cheese fortification using water-in-oil-in-water double emulsions as carrier for water soluble nutrients. International Dairy Journal. 29(2): 107-114.
  66. Huang, H., Wang, D., Belwal, T., Dong, L., Lu, L., Zou, Y., Li, L., Xu, Y., Luo, Z. 2021. A novel W/O/W double emulsion co-delivering brassinolide and cinnamon essential oil delayed the senescence of broccoli via regulating chlorophyll degradation and energy metabolism. Food Chemistry. 356: 129704.
  67. Castel, V., Rubiolo, A.C., Carrara, C.R. 2017. Droplet size distribution, rheological behavior and stability of corn oil emulsions stabilized by a novel hydrocolloid (Brea gum) compared with gum arabic. Food Hydrocolloids. 63: 170-177.
  68. Li, K., Woo, M.W., Patel, H., Selomulya, C. 2017. Enhancing the stability of protein-polysaccharides emulsions via Maillard reaction for better oil encapsulation in spray-dried powders by pH adjustment. Food Hydrocolloids. 69: 121-131.