the effect of polyphenols extragted from ol ive tarom cultivar to increase the oxidative stability of row and cooked refrigerated meat

Document Type : Complete scientific research article

Authors

1 Department of Food Science and Technology, Islamic Azad University of Science and Research of Tehran, Tehran, Iran

2 Department of Food Science and Technology, Islamic Azad University, Shahrekord

Abstract

Background:According to consumer awareness of the disadvantages of synthetic antioxidants, today the use of polyphenols in the mill olive wastewater (MOW) as a natural antioxidant in food is highly regarded. Therefore, this study aimed to identify the type and amount of polyphenols present in mill olive wastewater of Tarom culture and then use it as a natural antioxidant in oxidative stability of raw and cooked beef.
Material and Method: For this purpose, olive fruits were prepared manually from Tarom city, after oiling and milling, the type and amount of polyphenols were determined by the HPLC method in mill olive wastewater . The extract added to raw and cooked beef (cooked in water for 30 min) at 10, 20 and 30%. Thiobarbituric Acid, Peroxide value and conjugated dienes were measured for sample oxidative stability. Finally, the samples evaluated for overall acceptability.
Finding: The type and amount of polyphenols present in OMW were Oleuropein (20.42 ug/g), fluoric acid (17/91 ug /‌g), p-coumaric acid (11/76 ug /‌g), caffeic acid (3.75 ug‌/‌g), apigenin (2.5 ug‌/-g), and cinnamic acid (1.75 ug /‌g). Analysis of peroxide value as primary oxidation products showed a significant difference between the raw and cooked groups and an increasing trend observed in both groups (Treatments containing polyphenolic extract showed less increase compared to the control sample) which were due to decomposition of primary hydroperoxides into volatile and inactive compounds was lower. Evaluation of thiobarbituric acid as a secondary oxidation product in raw and cooked meat showed an increasing trend over storage time in both groups. The amount of thiobarbituric acid in cooked meat samples was higher than the raw counterpart (P<0.05), probably was due to lipid peroxidation and increased levels of malondialdehyde. The results of conjugates dienes analysis showed an increasing trend in both groups with an increased shelf life. The lower increase of this parameter in the treatment samples containing mill olive wastewater showed that the mill olive wastewater as antioxidant increased oxidative resistance compared to the control group. Overall acceptance of treatments showed that all cooking treatments had higher scores than their raw counterparts (p <0.05), and samples contain 30% extract) achieved the highest score for up to 8 days.
Result: research has shown that the rate of lipid oxidation in cooked meat is higher than raw meat because it changes the cooking process of antioxidant compounds, damages the cell structure, and exposes the lipid membrane to the environment.

Keywords


  1. Alu’datt, M.H., Alli, I., Ereifej, K., Alhamad, M., Al-Tawaha, A.R. and Rababah, T. 2010. Optimizations, characterization and quantification of phenolic compounds in olive cake. Food Chemistry. 123: 1.117-122.
  2. Amaral, A.B., Silva, M.V.D., and Lannes, S.C.D.S. 2018. Lipid oxidation in meat: mechanisms and protective factors–a review. Food Science and Technology. 38: 1.1-15.
  3. Association of Official Analytical Chemists, 1995. Official methods of analysis (16th Edition). Washington, DC, USA: Association of Official Analytical Chemists.
  4. Azaizeh, H., Halahlih, F., Najami, N., Brunner, D., Faulstich, M., and Tafesh, A. 2012. Antioxidant activity of phenolic fractions in olive mill wastewater. Food Chemistry. 134: 2226-2234.
  5. Balzan, S., Taticchi, A., Cardazzo, B., Urbani, S., Servili, M., Di Lecce, G., Zabalza, I., EnricoNovelli, M.,and Fasolato, L. 2017. Effect of phenols extracted from a by-product of the oil mill on the shelf-life of raw and cooked fresh pork sausages in the absence of chemical additives. LWT-Food Science and Technology. 85: Part A.89-95.
  6. Branciari, R., Ranucci, D., Miraglia, D., Urbani, S., Esposto, S. and Servili, M. 2015. Effect of dietary treatment with olive oil by-product (olive cake) on physicochemical, sensory and microbial characteristics of beef during storage. Food Safety.4: 4.5496.
  7. Chaijan, M. 2008. Lipid and myoglobin oxidations in muscle foods. Songklanakarin J. of Science & Technology. 30: 1.47-53.
  8. De Azevedo Gomes, H., da Silva, E.N., do Nascimento, M.R.L., and Fukuma, H. T. 2003. Evaluation of the 2-thiobarbituric acid method for the measurement of lipid oxidation in mechanically deboned gamma irradiated chicken meat. Food Chemistry. 80: 3.433-437.
  9. DeJong, S., and Lanari, M.C. 2009. Extracts of olive polyphenols improve lipid stability in cooked beef and pork: Contribution of individual phenolics to the antioxidant activity of the extract. Food Chemistry.116: 4.892-897.
  10. De Marco, E., Savarese, M., Paduano, A. and Sacchi, R. 2007. Characterization and fractionation of phenolic compounds extracted from olive oil mill wastewaters. Food Chemistry. 104: 2.858-867.
  11. Dermeche, S., Nadoura, M., Larroche, C., Moulti-Mati, F., and Michaud, P. 2013. Olive mill wastes: biochemical characterizations and valorization strategies. Process Biochemistry. 48: 10. 1532-1552.
  12. El-Abbassi, A., Kiai, H. and Hafidi, A. 2012. Phenolic profile and antioxidant activities of olive mill wastewater. Food Chemistry. 132: 1.406-412.
  13. El-Kalyoubi, M. H., Khallaf, M.M., Zahran, H.A, and Abdel-Razek, A.G. 2015. Potential application of olive mill waste extracts as natural antioxidants for improving the oxidative stability of fatty foods. Current Science International. 3. 313-319.
  14. Feiner, G. 2006. Meat products handbook: Practical science and technology. Elsevier. pp: 501-553.
  15. Galanakis, C. M. 2018. Phenols recovered from olive mill wastewater as additives in meat products. Trends in food science & technology. 79. 98-105.
  16. Gotoh, N., and Wada, S. 2006. The importance of peroxide value in assessing food quality and food safety. Oil Chemists' Society. 83: 5. 473-474.
  17. Hashempour, A., Ghazvini, R.F., Bakhshi, D. and Sanam, S.A. 2010. Fatty acids composition and pigments changing of virgin olive oil (Olea europea L.) in five cultivars grown in Iran. Australian J. of Crop Science. 4: 4.258-263.
  18. Hayes, J. E., Stepanyan, V., Allen, P., O’grady, M. N., and Kerry, J. P. 2011. Evaluation of the effects of selected plant-derived nutraceuticals on the quality and shelf-life stability of raw and cooked pork sausages. LWT-Food Science and Technology. 44: 1.164-172.
  19. Institute of Standards and Industrial Research of Iran. 2008. Olive oil - Ultraviolet extinction coefficient measurement- Test method. National Iranian Standard No. 10503. First revision. (In Persian).
  20. Institute of Standards and Industrial Research of Iran. 2012. Olive oil - Characteristics and test methods. National Iranian Standard No. 1446. Second revision. (In Persian).
  21. Institute of Standards and Industrial Research of Iran. 2013. Olive oil - Identification and determination of biophenols by high performance liquid chromatography (HPLC) test method. National Iranian Standard No. 16323. First Edition. (In Persian).
  22. Institute of Standards and Industrial Research of Iran. 2018. Animal and vegetable oils and fats - peroxide measurement by iodometric method - End point determination by ocular method. National Iranian Standard No. 4179. Second revision. (In Persian).
  23. Jaafarpour, S.A and Shukri, M. 2014. Biochemical, tissue, and sensory evaluation of combined red and surimi meat burger of Common Carp during freezing. J. of Food Industry Research. 27: 1.37-58. (In Persian).
  24. Jebeli Javan, A., Saberi, M., Javaheri, A.S. and Ghaffari, S. 2014. The Effect of adding Aloe Vera extract to broiler chicken diet based on chicken fillet peroxidation in freezing condition. J. of Veterinary Research. 68: 3. 240-233.
  25. Julie, K. 2007. Factors affecting poultry meat quality”. The University of Georgia. College of Agricultural and Environmental Science. Cooperative Extension Service. 33-60
  26. Kostaki, M., Giatrakou, V., Savvaidis, I. N., and Kontominas, M. G. 2009. Combined effect of MAP and thyme essential oil on the microbiological, chemical and sensory attributes of organically aquacultured sea bass (Dicentrarchus labrax) fillets. Food microbiology. 26: 5. 475-482.
  27. Lee, M.A., Choi, J.H., Choi, Y.S., Han, D.J., Kim, H.Y., Shim, S.Y., Chung, H.K. and Kim, C.J., 2010. The antioxidative properties of mustard leaf (Brassica juncea) kimchi extracts on refrigerated raw ground pork meat against lipid oxidation. Meat Science. 84: 3.498-504.
  28. Leouifoudi, I., Zyad, A., Amechrouq, A., Oukerrou, M. A., Mouse, H. A., and Mbarki, M. 2014. Identification and characterisation of phenolic compounds extracted from Moroccan olive mill wastewater. Food Science and Technology. 34: 2. 249-257.
  29. Levermore, R. 2004. Rancidity in fresh and stored pork products. Meat International. 14. 16-18.
  30. Luciano, G., Pauselli, M., Servili, M., Mourvaki, E., Serra, A., Monahan, F. J., and Mele, M. 2013. Dietary olive cake reduces the oxidation of lipids, including cholesterol, in lamb meat enriched in polyunsaturated fatty acids. Meat Science. 93: 3. 703-714.
  31. Mojerloo, Z., Elhami Rad, H., and Najafi, A. 2016. Antioxidant effect of ethanolic extract of olive meal on oxidative stability of soybean oil in comparison with some chemical antioxidants. J. of Innovation in Food Science and Technology. 8: 3. 15-23. (In Persian).
  32. Mokhtar, S.M., Youssef, K.M. and Morsy, N.E. 2014. The effects of natural antioxidants on colour, lipid stability and sensory evaluation of fresh beef patties stored at 4 ℃. Agroalimentary Processes and Technologies. 20: 3. 282-292.
  33. Muíño, I., Díaz, M.T., Apeleo, E., Pérez-Santaescolástica, C., Rivas-Cañedo, A., Pérez, C. and de la Fuente, J. 2017. Valorisation of an extract from olive oil waste as a natural antioxidant for reducing meat waste resulting from oxidative processes. J. of Cleaner Production. 140. 924-932.
  34. Pourkhalili, A., Mirlohi, M., Rahimi, E. and Hojatoleslami, M. 2013. Effect of conventional cooking methods on lipid oxidation indices in lamb meat. J. of Food Hygiene. 2: 1. 19-30.
  35. Rahman, M.H., Hossain, M.M., Rahman, S. M. E., Amin, M. R., and Oh, D.H. 2015. Evaluation of physicochemical deterioration and lipid oxidation of beef muscle affected by freeze-thaw cycles. Korean J. for food science of animal resources. 35: 6. 772-782.
  36. Roselló-Soto, E., Barba, F.J., Parniakov, O., Galanakis, C.M., Lebovka, N., Grimi, N., and Vorobiev, E. 2015. High voltage electrical discharges, pulsed electric field, and ultrasound assisted extraction of protein and phenolic compounds from olive kernel. Food and Bioprocess Technology. 8: 4. 885-894.
  37. Sampaio, G. R., Saldanha, T., Soares, R. A.M., and Torres, E.A.F.S. 2012. Effect of natural antioxidant combinations on lipid oxidation in cooked chicken meat during refrigerated storage. Food Chemistry. 135: 3.1383-1390
  38. Weber, J., Bochi, V.C., Ribeiro, C.P., Victório, A.D.M., and Emanuelli, T. 2008. Effect of different cooking methods on the oxidation, proximate and fatty acid composition of silver catfish (Rhamdia quelen) fillets. Food Chemistry. 106:1. 140-146.
  39. Widayaka, K., Setyawardani, T., and Sumarmono, J. 2001. The effect of storage and cooking on lipid oxidation of raw and cooked beef and goat meat. Asia Pacific J. of Clinical Nutrition. 10: 4. 548.556.
  40. Zahid, M.A., Seo, J.K., Park, J.Y., Jeong, J.Y., Jin, S.K., Park, T.S. and Yang, H.S. 2018. The effects of natural antioxidants on protein oxidation, lipid oxidation, color, and sensory attributes of beef patties during cold storage at 4℃. Korean J. for food science of animal resources. 38: 5. 1029-1042.
  41. Roila, R., Valiani, A., Miraglia, D., Ranucci, D., Forte, C., Trabalza-Marinucci, M., and Branciari, R. 2018. Olive mill wastewater phenolic concentrate as natural antioxidant against lipid-protein oxidative deterioration in chicken meat during storage. Italian J. of food safety. 7: 3.148-152.