Antioxidant effect of lavender extracts and essential oil phytochemical compounds on the stability of sunflower oil

Document Type : Complete scientific research article

Authors

1 Department of Biology, Falavarjan branch, Islamic Azad University, Isfahan, Iran

2 Department of Biochemistry, Falavarjan branch, Islamic Azad University, Isfahan, Iran

Abstract

The onset of some diseases is due to the increased free radicals produced during normal body metabolism. To confront these radicals, compounds known as antioxidants have been identified. Antioxidants that there are in fruits and vegetable, prevent the free radicals from doing activity and damage the vital cells. Antioxidants prevent oxidation of oils. However, with regard to synthetic antioxidants may cause undesirable effects in the body, the preparation and production of natural antioxidants is necessary. One of the plants with high antioxidant capacity is Lavandula officinalis L. belongs to the Lamiaceae.
Materials and methods: The fresh shoots of Lavender were harvested from the Agricultural and Natural Resources Research Center of Isfahan in June 2017 and dried in a dark place and room temperature. Essential oil was extracted by water distillation method using Clevenger's apparatus. Extract of water, methanol (80% methanol) and hexane (hexane 95%) was prepared by maceration method. The phenols, flavonoids and terpenoids amount of extracts was determined respectively by Folin-Ciocalteu, aluminum chloride and separation colorimetric in ether methods. The antioxidant capacity of each extract was analyzed by DPPH method. Essential oil composition of lavender were determined by GC-MS. The effect of extracts on delaying oxidative degradation in Purified sunflower oil was determined by measuring the peroxide index, anisidine index, totox index and oxidation resistance time of the samples.
Results: Phenol, flavonoid and terpenoid contents of methanol extract was higher than the other two extractions. Free radical scavenging of methanol and water extracts were higher than hexane extract and were similar to BHT. Inhibition percent of hexane extract was less than the other extracts. The performance of aqueous extracts was higher than other extracts. The major compounds identified by GC-MS are linalol, linal acetate, boronol, camphor and geranyl acetate. Methanolic extracts had the lowest levels of peroxide index, anisidine index and totox index and the highest oxidation stability time. Methanol extract had the best effect in preventing the oxidation of sunflower oil. The effect of the aqueous extract and essential oil were similar to the synthetic antioxidant.
Coclution: By comparing the three extracts it can be concluded that methanol is the best solvent for the extraction of phenol, flavonoids and terpenoid. On the other hand, methanolic extract had a higher inhibitory effect than other extracts. The methanol extract with high anti-radical effect can be replaced by BHT in sunflower oil in the food industry. The aqueous extracts and essential oils of this plant can also be used as antioxidants similar to synthetic antioxidants.

Keywords

References

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Food Processing and Preservation Journal
Volume 13, Issue 1
September 2021
Pages 115-123

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