Inactivation of Pectin methylesterase in carrot-kiwi juice during thermal processing

Document Type : Complete scientific research article

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Abstract

Background and objectives: In this study, the mixture of carrot and kiwi juice is introduced as a new food product with high nutritional value. Turbidity is considered as a desirable property in carrot - kiwi juice that is influenced by the presence of released pectin into the extraction. Pectin methylesterase (PME) desterified the pectin that resulted in the turbidity loss in the juice. Thermal processing is known as the most common method to PME inactivation. The aim of this study is to investigate the PME inactivation during carrot - kiwi juice thermal processing at different temperature.
Materials and methods: To study the inactivation of pectin methylesterase, at first the carrot - kiwi juice was heated at 60, 70. 80 and 90 ℃ in different time (proportional to the temperature). Using a thermocople, the changes in the temperature of the sample were recorded and reported in a second. During thermal processing, the activity of the enzyme was measured according to Kimball (1991) method. D-value, Z-value and also thermodynamic parameters of the thermal destruction of PME were calculated.
Results: Based on the obtained results, Z-value of heat labile and stable isoforms of PME in the juice were estimated 17.85℃ and 22.27℃, respectively. The required activation energy to inactivate the heat labile and stable isoforms of PME in the carrot - kiwi juice were calculated 356.83 kJ/mol and 257.17 kJ/mol, respectively. Enthalpy for inactivation of the heat labile (354.1 to 353.8 kJ.mol-1) and stable (254.4 to 254.1 kJ.mol-1) isoforms were estimated. During the carrot - kiwi juice processing, the entropy of the inactivation of the heat labile and stable isoforms were calculated 0.79 to 0.73 K-1.kJ.mol-1 and 0.47 to 0.43 K-1.kJ.mol-1, respectively. Also, the free energy related to the inactivation of the heat labile (91.1 to 87.3 kJ.mol-1) and stable (97.9 to 97.0 kJ.mol-1) isoforms of PME was measured. These results represent the effect of temperature on the protein structure of the enzyme. In addition, the effectiveness percentage of the come up time (CUT) on inactivation of the heat labile (55-22%) and stable (66-22%) isoforms of PME in the carrot - kiwi juice were computed.
Conclusion: The overheating of the product and also loss of nutritional value, organoleptic and qualitative properties and also energy wasting could be prevented by reducing the required thermal processing temperature of the carrot juice by its combination with the kiwi fruit that resulted in pH adjustment and also study the effect of CUT on the PME inactivation.

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References


1. Aghajanzadeh, S., Kashaninejad, M., and Ziaiifar, A.M. 2016. Effect of infrared heating on
degradation kinetics of key lime juice physicochemical properties. Innovative Food Science
& Emerging Technologies, 38, 139-148.
2. Aghajanzadeh, S., Ziaiifar, A.M., Kashaninejad, M., Maghsoudlou, Y., and Esmailzadeh, E.
2016. Thermal inactivation kinetic of pectin methylesterase and cloud stability in sour
orange juice. Journal of Food Engineering, 185: 72-77.
3. Alasalvar, C., Grigor, JM., Zhang, D., Quantick, P. C., and Shahidi, F. 2001. Comparison of
volatiles, phenolics, sugars, antioxidant vitamins, and sensory quality of different colored
carrot varieties. Journal of Agricultural and Food Chemistry. 49(3): 1410-1416.
4. Alothman ,M., Bhat, R., and Karim, A. 2009. UV radiation-induced changes of antioxidant
capacity of fresh-cut tropical fruits. Innovative Food Science & Emerging Technologies. 10:
4. 512-516.
5. AOAC. 2012. Association of Official Analytical Chemists. Official methods of analysis of
the Association of Official Analytical Chemists, Washington, DC: AOAC, Ed. 19.
6. Ball, C.O. 1923. Thermal process time for canned food. Published by the National Research
Council of The National Academy of Sciences.
7. Bordenave, M., and Goldberg, R. 1993. Purification and characterization of pectin
methylesterases from mung bean hypocotyl cell walls. Phytochemistry. 33: 5. 999-1003.
8. Byers, T., and Guerrero, N. 1995. Epidemiologic evidence for vitamin C and vitamin E in
cancer prevention. The American Journal of Clinical Nutrition. 62 (2): 1385-1392.
نشریه فرآوری و نگهداری مواد غذایی جلد ) ،(11شماره 1398 ،1
114
9. Cameron, R.G., Baker, R.A., and Grohman, K. 1998. Multiple forms of pectinmethylesterase
from citrus peel and their effects on juice cloud stability. Journal of Food Science. 63(2):
253-256.
10.Chen, C., and Wu, M. 1998. Kinetic models for thermal inactivation of multiple
pectinesterases in citrus juices. Journal of Food Science. 63 (5): 747-750.
11.Ercan, S.Ş., and Soysal, Ç. 2011. Effect of ultrasound and temperature on tomato peroxidase.
Ultrasonics Sonochemistry. 18(2): 689-695.
12.Espachs-Barroso, A., VanLoey, A., Hendrickx, M., and Martín-Belloso, O. 2006.
Inactivation of plant pectin methylesterase by thermal or high intensity pulsed electric field
treatments. Innovative food Science & Emerging Technologies. 7(1): 40-48.
13.Eyring, H. 1935. The activated complex and the absolute rate of chemical reactions.
Chemical Reviews. 17(1): 65-77.
14.Hsieh, C.-W., and Ko, W.-C. 2008. Effect of high-voltage electrostatic field on quality of
carrot juice during refrigeration. LWT-Food Science and Technology. 41(10): 1752-1757.
15.Kertesz, Z.I. 1951. The pectic substances. Vol. 1: Interscience Publishers New York.
16.Kimball, D.A. 1991. Citrus processing-quality control and technology, New York: Van
Nostrand Reinhold, 117-243.
17.Kuldiloke, J. 2002. Effect of ultrasound, temperature and pressure treatments on enzyme
activity and quality indicators of fruit and vegetable juices. PhD TU-Berlin.
18.Ly‐Nguyen, B., Loey, AV., AM., Fachin, D., Verlent, I., Duvetter, T., Vu, ST., Smout, C.,
and Hendrickx, ME. 2002. Strawberry Pectin Methylesterase (PME): Purification,
Characterization, Thermal and High‐Pressure Inactivation. Biotechnology Progress. 18(6):
1447-1450.
19.Ly‐Nguyen, B., Loey, AV., Smout, C., ErenÖzcan, S., Fachin, D., Verlent, I., Truong, S.V.,
Duvetter, T., and Hendrickx, ME. 2003. Mild‐Heat and High‐Pressure Inactivation of Carrot
Pectin Methylesterase: A Kinetic Study. Journal of Food Science. 68(4): 1377-1383.
20.Marques-Vidal, P., Ravasco, P., and Camilo, M.E. 2006. Foodstuffs and colorectal cancer
risk: a review. Clinical Nutrition. 25(1): 14-36.
21.Marshall, M., Marcy, J., and Braddock, R.J. 1985. Effect of total solids level on heat
inactivation of pectinesterase in orange juice. Journal of Food Science. 50(1): 220-222.
22.Marx, M., Schieber, A., and Carle, R. 2000. Quantitative determination of carotene
stereoisomers in carrot juices and vitamin supplemented (ATBC) drinks. Food Chemistry.
70(3): 403-408.
23.Patterson, M.F., Patterson, M.F., McKay, AM., Connolly, M., and Linton, M. 2012. The
effect of high hydrostatic pressure on the microbiological quality and safety of carrot juice
during refrigerated storage. Food Microbiology, 30(1): 205-212.
24.Polydera, A., Galanou, E., Stoforos, N.G., and Taoukis, PS. 2004. Inactivation kinetics of
pectin methylesterase of greek Navel orange juice as a function of high hydrostatic pressure
and temperature process conditions. Journal of Food Engineering. 62(3): 291-298.
25.Rakcejeva, T., Augspole, I., Dukalska, L., and Dimins, F. 2012. Chemical composition of
variety ‘Nante’hybrid carrots cultivated in Latvia. J World Acad Sci Eng Technol. 6(4): 901-
907.
26.Rivas, A., Rivas, A., Rodrigo, D., Martínez, A., Barbosa-Cánovas, G.V., and Rodrigo, M.
2006. Effect of PEF and heat pasteurization on the physical–chemical characteristics of
blended orange and carrot juice. LWT-Food Science and Technology. 39(10): 1163-1170.
27.Rodriguez-Amaya, D. 1993. Stability of carotenoids during the storage of foods.
Developments in food Science.
28.Rouse, A. 1953. Distribution of pectinesterase and total pectin in component parts of citrus
fruits. Food Technology. 7(9): 360-362.
29.Rouse, A., and Atkins, C. 1955. Pectinesterase and pectin in commercial citrus juices as
determined by methods used at the Citrus Experiment Station.
30.Rothschild G, Vanvliet C and Karsenty A, 1975. Pasteurization conditions for juices and
comminuted products of Israeli citrus fruits. Food Technology, 10: 29-38.
صدف کنودان و همکاران
115
31.Sensoy, I., Zhang, Q.H., and Sastry, S.K. 1997. Inactivation kinetics of Salmonella dublin by
pulsed electric field. Journal of Food Process Engineering. 20(5): 367-381.
32.Sharma, K.D., Karki, S., Thakur, N. S., and Attri, S. 2012. Chemical composition, functional
properties and processing of carrot—a review. Journal of Food Science and Technology.
49(1): 22-32.
33.Sio, F., Palmieri, A., Servillo, L., Giovane, A., and Castaldo, D. 2001. Thermoresistance of
pectin methylesterase in Sanguinello orange juice. Journal of Food Biochemistry. 25(2): 105-
115.
34.Tajchakavit, S., and Ramaswamy, H. 1997. Thermal vs. Microwave Inactivation Kinetics of
Pectin Methylesterase in Orange Juice Under Batch Mode Heating Conditions. LWT-Food
Science and Technology. 30(1): 85-93.
35.Tanaka, A., and Hoshino, E. 2003. Similarities between the thermal inactivation kinetics of
Bacillus amyloliquefaciensα‐amylase in an aqueous solution of sodium dodecyl sulphate and
the kinetics in the solution of anionic‐phospholipid vesicles. Biotechnology and Applied
Biochemistry. 38(2): 175-181.
36.Tiwari, B., Muthukumarappan, K., O'donnell, CP., and Cullen, PJ. 2009. Inactivation
kinetics of pectin methylesterase and cloud retention in sonicated orange juice. Innovative
Food Science & Emerging Technologies. 10(2): 166-171.
37.Vandresen, S., Quadri, MG., de Souza, JA., and Hotza, D. 2009. Temperature effect on the
rheological behavior of carrot juices. Journal of Food Engineering. 92(3): 269-274.
38.Versteeg, C., Rombouts, FM., Spaansen, CH., and Pilnik, W. 1980. Thermostability and
orange juice cloud destabilizing properties of multiple pectinesterases from orange. Journal
of Food Science. 45(4): 969-971.
39.Vikram, V., Ramesh, M., and Prapulla, S. 2005. Thermal degradation kinetics of nutrients in
orange juice heated by electromagnetic and conventional methods. Journal of Food
Engineering. 69(1): 31-40.
40.Wang, T., and Gleave, A.P. 2012. Applications of Biotechnology in Kiwifruit (Actinidia).:
Intech Open Access Publisher.
41.Wicker, L., and Temelli, F. 1988. Heat inactivation of pectinesterase in orange juice pulp.
Journal of Food Science. 53(1): 162-164.
42.Yeom, H., Zhang, Q., and Chism, G. 2002. Inactivation of pectin methyl esterase in orange
juice by pulsed electric fields. Journal of Food Science. 67(6): 2154-2159.
43.Zhou, L., Wang, Y., Hu, X., Wu, J., and Liao, X. 2009. Effect of high pressure carbon
dioxide on the quality of carrot juice. Innovative Food Science & Emerging Technologies.
10(3): 21-327.
Food Processing and Preservation Journal
Volume 11, Issue 1
July 2019
Pages 103-116

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