Evaluation of Lauroyl-l-arginine ethyl ester release and antibacterial properties of bio active zein films

Background and objectives: Lauroyl arginine ethyl ester (LAE) has been focused as a new active compound with antimicrobial property for food preservative. Because of environmental concerns, the incorporation of preservatives into biodegradable films is more suitable than their incorporation into plastic films.The aim of this work was to develop antimicrobial including LAE in bio zein matrix and evaluation of releasing of lauroyl arginine ethyl ester in the food models solutions and also antimicrobial properties produce films.
Materials and methods: Active zein films were made incorporating 10% LAE in the film forming solution by casting method. The kinetics of LAE mass transport from bioactive films zein containing 10% LAE into two food simulants (10% ethanol and 3% acetic acid) were evaluated by using high performance liquid chromatography at 4, 22, and 37 °C. Effect of food simulants on the structure of the zein film was evaluated by SEM. Antimicrobial effect of active zein films with 10% LAE was investigated against E. coli and L. Inoccua at 4 and 37 °C.