Microencapsulation of Q10 by complex coacervation method

Document Type : Complete scientific research article

Authors

1 Isfahan university of technology

2 Isfahan University of Technology

Abstract

Background and objectives: CoQ10 is a lipophylic natural antioxidant with fundamental role in mitochondria bioenergetics. The positive effect of CoQ10 in therapy of many diseases is due to its antioxidant and bioenergetics properties. Reduction of endogenous synthesis of CoQ10 by the ageing, insufficient amount in the food and several health benefits of CoQ10 have led to food fortification. The objective of this study was to microencapsulate CoQ10 by complex coacervation method and determinate of optimum mixture component (β-lactoglobulin (β-lg), Arabic Gum (AG), Oil containing CoQ10 and water) to achieve maximum value of microencapsulation efficiency (ME) and payload. Due to temperature sensitive characteristic of CoQ10, Gelatin was replaced with β-lg and combination of β-lg and AG were used to microencapsulation of Oil containing CoQ10.
Materials and methods: At first CoQ10 powder and olive oil were poured in opaque tube and stirred in incubator shaker for 24h. Then solutions of β-lg and GA were prepared at 1-4% (w/w), by dispersing the required amount of them in deionized water and microcapsules were formed by adding oil phase containing CoQ10 to the β-lg solution and preparation of emulsion, adding the GA solution and adjusting pH to 4. After separation of microcapsules freeze dryer was used to dehydration of them, and Morphology of CoQ10 microcapsules was examined using a Scanning Electron Microscope (SEM) and an optical microscope. The particle size and the particle size distribution of moist microcapsules were determined using a particle size analyzer. In the next stage it was used from n-hexane to extracting oil and extracted oil was dissolved in 1,4-dioxane and analyzed by a high performance liquid chromatography (HPLC) system, and microencapsulation efficiency was calculated for microcapsules before and after freeze drying. At the end the effect of oven drying and defrost on microencapsulation efficiency was studied for 5 formulations.
Results: ME values before drying for all formulations except one sample (containing: 4%β-lg, 4%AG, 5%Oil and 87% water) were in the range of 94.05-99.01%. Variation in ME and payload values after freeze drying was in the range of 34.91-92.45 and 24.78-83.75%, respectively and sample containing 2.5% (w/w) β-lg and 2.5% (w/w) AG and 5 % (w/w) oil obtained the highest ME value, an homogeneous particle size distribution and had a smooth and free of pores surface.
Conclusion: Based on the results, formulation containing 2.5% (w/w) β-lg and AG and 5 % (w/w) oil can be successfully used to microencapsulation of CoQ10.

Keywords

Main Subjects

References

Alvim, I.D., and Grosso, C.R.F. 2010. Microparticles obtained by complex coacervation: influence of the type of reticulation and the drying process on the release of the core material. Food science and Technology. 30: 1069-1076.
Bule, M.V., Singhal, R.S., and Kennedy, J.F. 2010. Microencapsulation of ubiquinone-10 in carbohydrate matrices for improved stability. Carbohydrate polymers. 82: 1290-1296.
Burgess, D.J., and Carless, J.E. 1984. Microelectrophoretic studies of gelatin and acacia for the prediction of complex coacervation. . Journal of Colloid and Interface Science. 98: 1–8.
Burgess, D.J., and Ponsart, S. 1998. Beta-Glucuronidase activity following complex coacervation and spray drying microencapsulation. Journal of Microencapsulation. 15: 569-579.
Fir, M.M., Luka, M., Mirko, P., and Andrej, S. 2009. Property Studies of Coenzyme Q10–Cyclodextrins complexes. Acta Chimica Slovenica. 56: 885-891.
Junyaprasert, V.B., Mitrevej, A., Sinchaipanid, N., Boonme, P., and Wurster, D.E. 2001. Effect of process variables on the microencapsulation of vitamin A palmitate by gelatin–acacia coacervation. Drug Development and Industrial Pharmacy. 27: 561–566.
Mendanha, D.V., Ortiz, S.E.M., Favaro-Trindade, C.S., and Mauri, A. 2009. Monterrey-Quintero and M. Thomazini. Microencapsulation of casein hydrolysate by complex coacervation with SPI/pectin. Food Research International. 42: 1099–1104.
Nepal, P.R., Han, H.K., and Choi, H.K. 2010. Enhancement of solubility and dissolution of Coenzyme Q10 using solid dispersion formulation. International Journal of Pharmaceutics. 383: 147–153.
Omar, K.A., Shan, L., Zou, X., Song, Z., and Wang, X. 2009. Effects of two emulsifiers on yield and storage of flax oil powder by response surface methodology. Pakistan Journal of Nutrition. 8: 1316-1324.
10. Pravst, I., Zmitek, K., and Zmitek, J. 2010. Coenzyme Q10 Contents in Foods and Fortification Strategies. Food Science and Nutrition. 50: 269-280.
11. Quispe-Condori, S., Marleny, S., and Temelli, T. 2011. Microencapsulation of flax oil with zein using spray and freeze drying. Food Science and Technology. 67: 1-8.
Schmitt, C., Sanchez, C., Despond, S., Renard, D., Thomas, F., and Hardy, J. 1999. Complex coacervation between b-lactoglobulin and acacia gum in aqueous medium. Food Hydrocolloid. 13: 483-496.
Tamjidi, F., Nasirpour, A., and Shahedi, M. 2013. Mixture design approach for evaluation of fish oil microencapsulation in gelatin-acacia gum coacervates. International Journal of Polymeric Material. 62: 444-449.
Weinbreck, F., Minor, M., and Kruif, C.G. 2004. Microencapsulation of oils using whey protein/gum Arabic coacervates.  Journal of Microencapsulation. 21: 667–679.
Yeo, Y., Bellas, E., Firestone, W., Langer, R., and Kohane, D.S. 2005. Complex coacervates for thermally sensitive controlled release of flavor compounds. Journal of Agricultural and Food Chemistry. 53: 7518-7525.
Food Processing and Preservation Journal
Volume 10, Issue 1
July 2018
Pages 1-16

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